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Diagnostic Techniques

» What is the most sensitive office laboratory test for diagnosing dermatophyte infections of the skin?
» How is a KOH examination performed?
» What laboratory tests are useful for diagnosing tinea capitis?
» What is a Wood’s light or lamp? How is it useful in skin diseases?
» Name common culture media used for isolating dermatophytes.
» Describe a simple test for tinea versicolor other than a KOH preparation.
» What is a Tzanck preparation or smear?
» What is the best method of diagnosing scabies?
» How do you diagnose mite bites acquired from an animal?
» How do you diagnose lice infestation?
» What is the diagnostic test of choice for a patient presenting with a suspected syphilitic chancre on his penis?
» How is secondary syphilis diagnosed?
» How long do serologic tests for syphilis remain positive?
» In patients with symptomatic gonococcal urethritis, how efficacious is a Gram stain of the exudate in comparison to a culture utilizing selective media for gonococcus?
» What is the best way to diagnose allergic contact dermatitis?
» How are patch tests applied?
» In what diseases is a skin biopsy helpful?
» When are shave biopsies indicated?
» What are the indications for punch biopsies?
» Describe the indications for an excisional or incisional biopsy.
» Define and describe direct immunofluorescence of the skin.
» Name some skin diseases in which DIF is helpful in making a diagnosis.
» How does indirect immunofluorescence of the skin differ from direct immunofluorescence of the skin?
» Is ELISA ever used for the diagnosis of immunobullous disease?
» How are bacterial skin cultures performed, and when are they useful?

 
 
 

How are bacterial skin cultures performed, and when are they useful?

Bacterial cultures are useful when active infection of the skin is suspected. Bacterial cultures demonstrate high yields in superficial infections such as impetigo, ecthyma, and infected ulcers, and lower yields in cellulitis. When culturing superficial infections, the involved area should first be cleaned with an alcohol pad and then thoroughly swabbed. A higher concentration of bacteria may be found at the point of maximal inflammation. The best results in cellulitis are obtained when the leading edge is injected with nonbacteriostatic saline using a 20-gauge needle mounted on a tuberculin syringe. The aspirate may be sent for culture while still in the syringe if it can be taken to the laboratory immediately, or the aspirated material may be submitted in a bacterial culturette.