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Diagnostic Techniques

» What is the most sensitive office laboratory test for diagnosing dermatophyte infections of the skin?
» How is a KOH examination performed?
» What laboratory tests are useful for diagnosing tinea capitis?
» What is a Wood’s light or lamp? How is it useful in skin diseases?
» Name common culture media used for isolating dermatophytes.
» Describe a simple test for tinea versicolor other than a KOH preparation.
» What is a Tzanck preparation or smear?
» What is the best method of diagnosing scabies?
» How do you diagnose mite bites acquired from an animal?
» How do you diagnose lice infestation?
» What is the diagnostic test of choice for a patient presenting with a suspected syphilitic chancre on his penis?
» How is secondary syphilis diagnosed?
» How long do serologic tests for syphilis remain positive?
» In patients with symptomatic gonococcal urethritis, how efficacious is a Gram stain of the exudate in comparison to a culture utilizing selective media for gonococcus?
» What is the best way to diagnose allergic contact dermatitis?
» How are patch tests applied?
» In what diseases is a skin biopsy helpful?
» When are shave biopsies indicated?
» What are the indications for punch biopsies?
» Describe the indications for an excisional or incisional biopsy.
» Define and describe direct immunofluorescence of the skin.
» Name some skin diseases in which DIF is helpful in making a diagnosis.
» How does indirect immunofluorescence of the skin differ from direct immunofluorescence of the skin?
» Is ELISA ever used for the diagnosis of immunobullous disease?
» How are bacterial skin cultures performed, and when are they useful?

 
 
 

When are shave biopsies indicated?

The choice of the biopsy technique requires knowledge of basic dermatology, most specifically, where in the skin the pathology is likely to be located. A shave biopsy is usually the most superficial of the skin biopsies and particularly useful when the lesion is in or close to the epidermis. A shave biopsy is best for pedunculated, papular, or otherwise exophytic lesions. It is particularly useful for diagnosis of basal cell and squamous cell carcinomas, seborrheic keratoses, warts, intradermal nevi, and pyogenic granulomas. Shave biopsies are often poor choices for biopsies of melanocytic lesions such as dysplastic nevi or melanoma. Unlike punch biopsies, shave biopsies only require a clean, nonsterile field and do not require sutures.