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Diagnostic Techniques

» What is the most sensitive office laboratory test for diagnosing dermatophyte infections of the skin?
» How is a KOH examination performed?
» What laboratory tests are useful for diagnosing tinea capitis?
» What is a Wood’s light or lamp? How is it useful in skin diseases?
» Name common culture media used for isolating dermatophytes.
» Describe a simple test for tinea versicolor other than a KOH preparation.
» What is a Tzanck preparation or smear?
» What is the best method of diagnosing scabies?
» How do you diagnose mite bites acquired from an animal?
» How do you diagnose lice infestation?
» What is the diagnostic test of choice for a patient presenting with a suspected syphilitic chancre on his penis?
» How is secondary syphilis diagnosed?
» How long do serologic tests for syphilis remain positive?
» In patients with symptomatic gonococcal urethritis, how efficacious is a Gram stain of the exudate in comparison to a culture utilizing selective media for gonococcus?
» What is the best way to diagnose allergic contact dermatitis?
» How are patch tests applied?
» In what diseases is a skin biopsy helpful?
» When are shave biopsies indicated?
» What are the indications for punch biopsies?
» Describe the indications for an excisional or incisional biopsy.
» Define and describe direct immunofluorescence of the skin.
» Name some skin diseases in which DIF is helpful in making a diagnosis.
» How does indirect immunofluorescence of the skin differ from direct immunofluorescence of the skin?
» Is ELISA ever used for the diagnosis of immunobullous disease?
» How are bacterial skin cultures performed, and when are they useful?

 
 
 

How do you diagnose lice infestation?


Microscopic examination of a hair shaft demonstrates an empty hair louse egg that is glued to the hair shaft. (Courtesy of James E. Fitzpatrick, MD.)
Fig. 3.7 Microscopic examination of a hair shaft demonstrates an empty hair louse egg that is glued to the hair shaft. (Courtesy of James E. Fitzpatrick, MD.)
Lice infestation is caused by Pediculus humanus capitis (head louse), P. humanus corporis (body louse), or Phthirus pubis (pubic louse). Lice can be identified by eye or by using a hand lens, but they can be very difficult to locate. If body lice infestation is suspected, examination of the seams of clothing is more likely to be diagnostic than is examining the skin. Lice will appear as brownish-gray specks. Head and pubic lice are more often seen in hairy areas and are often found with their mouth parts embedded in the skin with outstretched claws grasping hairs on either side. Usually more numerous are nits (eggs), which are white-gray oval structures smaller than 1 mm in size and are firmly attached to the hair shaft. Eggs that are near the junction of the hair shaft and the skin are indicative of active or recent infection. Since hair casts are white and may resemble nits, it is sometimes necessary to examine possible nits under the microscope to prove their identity (Fig. 3-7).